A student needed to prepare 500mL of 1X TAE buffer to run a QC gel. The stock solution in the lab is 5X TAE. What volumes of stock TAE and water are needed to prepare the 1X working TAE buffer?
Accepted Solution
A:
Answer:you need 100ml of 5X TAE and 400ml of water. Step-by-step explanation:You need to use a rule of three:[tex]C_1V_1=C_2V_2[/tex]where:[tex]\left \{ {{C_1= 5X} \atop {C_2=1X}} \right.[/tex]and[tex]\left \{ {{V_1 = V_{TAE}} \atop {V_2=500ml}} \right.[/tex]Therefore:[tex]V_{TAE} = \frac{V_2*C_2}{C_1}[/tex][tex]V_{TAE} = 100ml[/tex]Then just rest the TAE volume to the final Volume and you get the amount of water that you need to reduce the concentration.